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Old 04-26-2012, 12:32 PM   #15
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Originally Posted by Todd@Autopia View Post
From a percentage standpoint yes. But when discussing dilution rations 1:10 means dilute 10 parts of x with 1 part of y.

10:1 doesn't mean 100 percent/10 percent, it means 10 parts + 1 part. In terms of percentages it would be more like 90.91 percent/9.01 percent.

Dilluting something 10:1 gives it a dilution of 90.91:9.01.
OK. I give up. I just know that when I had to do serial dilutions on my cells it was 9 parts KK2 buffer and 1 parts cells. But I 'm honestly starting to get confused.
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Old 04-26-2012, 12:50 PM   #16
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OK. I give up. I just know that when I had to do serial dilutions on my cells it was 9 parts KK2 buffer and 1 parts cells. But I 'm honestly just starting to get confused.
so it's not just me!
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Old 04-26-2012, 12:58 PM   #17
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I get the overwhelming feeling you guys are just trying to get me to do math... I hate math
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Old 04-26-2012, 12:58 PM   #18
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DEFINITIONS:

Aliquot: a measured sub-volume of original sample.

Diluent: material with which the sample is diluted

Dilution factor (DF): ratio of final volume/aliquot volume (final volume = aliquot + diluent)

Concentration factor (CF): ratio of aliquot volume divided by the final volume (inverse of the dilution factor)


To calculate a dilution factor:

Remember that the dilution factor is the final volume/aliquot volume.

EXAMPLE: What is the dilution factor if you add 0.1 mL aliquot of a specimen to 9.9 mL of diluent?

The final volume is equal the the aliquot volume plus the diluent volume: 0.1 mL + 9.9 mL = 10 mL
The dilution factor is equal to the final volume divided by the aliquot volume: 10 mL/0.1 mL = 1:100 dilution (10 2)

The Concentration Factor for this problem = aliquot volume/final volume = 0.1/(0.1 + 9.9) = 0.01 or 10 -2 concentration


To prepare a desired volume of solution of a given dilution:

1. Calculate the volume of the aliquot: it is equal either to the final volume/dilution factor or the concentration factor x final volume

2. Calculate the volume of the diluent: which is equal to (the final volume - aliquot volume)

3. Measure out the correct volume of diluent, add the correct volume of aliquot to it, mix.

EXAMPLE: How would you prepare 20 mL of a 1:50 dilution?

Determine required aliquot by dividing final volume by dilution factor: 20 mL/50 = 0.4 mL sample
Subtract the aliquot volume from the final volume: 20 mL - 0.4 mL = 19.6 mL diluent
Measure out 19.4 mL diluent, add 0.4 mL sample to it, mix thoroughly
and here's a link o the site: http://biology.clc.uc.edu/fankhauser.../dilutions.htm
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Old 04-26-2012, 01:24 PM   #19
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I get the overwhelming feeling you guys are just trying to get me to do math... I hate math
So are you not going to chime in
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Old 04-26-2012, 01:45 PM   #20
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lol!
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Old 04-26-2012, 01:52 PM   #21
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lol!
You had me freaking out, thinking that been doing all of my serial solutions wrong
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Old 04-26-2012, 01:55 PM   #22
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You had me freaking out, thinking that been doing all of my serial solutions wrong
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Old 04-26-2012, 02:00 PM   #23
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i think dilution and mix ratios are getting confused, i don't think they are the same.

when you are talking about a mix, 1:10, it would be 1 part A plus 10 parts B, dillution would be 1 part A of 10 parts AB.


found online;

"Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)"

Last edited by Sleez; 04-26-2012 at 02:04 PM. Reason: spelling
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Old 04-26-2012, 02:11 PM   #24
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Quote:
Originally Posted by Sleez View Post
i think dilution and mix ratios are getting confused, i don't think they are the same.

when you are talking about a mix, 1:10, it would be 1 part A plus 10 parts B, dillution would be 1 part A of 10 parts AB.


found online;

"Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)"

Ding SDing Ding This is the winner. Ratio is just ratio
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Old 04-26-2012, 02:17 PM   #25
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Quote:
Originally Posted by Sleez View Post
i think dilution and mix ratios are getting confused, i don't think they are the same.

when you are talking about a mix, 1:10, it would be 1 part A plus 10 parts B, dillution would be 1 part A of 10 parts AB.


found online;

"Don't mix dilution and ratio...

Ratio means you have x parts of A and y parts of B.

x : y

Dilution refers to the total volume.

x : (x+y)"
Ok so for a dilution of 10:1 you'd have 1 part "x' and 10 parts "y". Correct?
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Old 04-26-2012, 02:20 PM   #26
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Originally Posted by nyrfan View Post
Ok so for a dilution of 10:1 you'd have 1 part "x' and 10 parts "y". Correct?

no, dilution would be 1 part A to 10 parts A+B, or 9 parts B for a total of 10 parts.

AFAIK
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Old 04-26-2012, 02:29 PM   #27
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no, dilution would be 1 part A to 10 parts A+B, or 9 parts B for a total of 10 parts.

AFAIK
That cannot be correct.

Look at the sample link below, the dilution is 10:1 of the product which will give you 11 total gallons. If is were done your way you only get 10 gallons.

http://www.detailedimage.com/Meguiar...P445/128oz-S1/
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Old 04-26-2012, 02:32 PM   #28
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just sayin', based on scientific definitions of dilution versus mix ratios.

your link explains a "mix" ratio, not a dilution.
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